1x minimal essential medium nonessential amino acids (mem neaa Search Results


non-essential amino acids (neaa  (Lonza)
90
Lonza non-essential amino acids (neaa
Non Essential Amino Acids (Neaa, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1x neaa  (DSMZ)
94
DSMZ 1x neaa
1x Neaa, supplied by DSMZ, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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neaa  (Corning Life Sciences)
90
Corning Life Sciences neaa
Neaa, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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neaa - by Bioz Stars, 2026-08
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1x mem non essential amino acids  (Quality Biological Inc)
86
Quality Biological Inc 1x mem non essential amino acids
1x Mem Non Essential Amino Acids, supplied by Quality Biological Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primordial germ cell like cell pgclc media  (Proteintech)
96
Proteintech primordial germ cell like cell pgclc media
a) GSEA of Stag3 KD RNA-seq data reveals a negative normalised enrichment score of -1.50 (adj p= 0.044) for the Spermatogenesis signature (see Methods). b) Relative mRNA expression of PGC regulators in siLuc and siStag3 mESCs. Data is shown as fold-change from UT and is from 9 independent biological replicates. Quantifications and statistical analysis as before. c) Representative brightfield (top row), epi-fluorescent (middle row) and FACS profiles of PRDM1-GFP mESCs at select timepoints during in vitro differentiation into PGCLCs. Scale bar is 200um. d) Relative mRNA expression of select mESC ( Nanog), EpiLC ( Dnmt3b ), <t>PGCLC</t> ( Prdm1, Dppa3 ) and cohesin ( Stag1, 2, 3 and Smc1b ) genes from various timepoints during PGCLC differentiation in vitro . EB GFP+ cells were FACS sorted at the timepoints shown and collected for qRT-PCR. Gene expression is relative to levels in mESCs. Data is from 3 independent biological replicates. e) (Top) Representative brightfield and epi-fluorescent images of DPPA3-GFP mESCs treated with siLuc or siStag3 at three timepoints of embryoid body (EB) differentiation. (Bottom) Representative FACS profile of DPPA3-GFP d6 EB treated with siLuc or siStag3. Scale bar is 200μm. f) Left, quantification of DPPA3-GFP GeoMean or right, the % of DPPA3-GFP+ cells assessed by FACS (relative to UT mESCs) at different stages of PGCLC differentiation and upon siRNA treatment. Data is from 4 independent biological replicates. Quantifications and statistical analysis as before. g) Relative mRNA expression of select genes in mESCs or d6 EB GFP+ cells in siLuc and siStag3 conditions. Data is shown as fold-change from UT and is from 3 independent biological replicates. Quantifications and statistical analysis as above.
Primordial Germ Cell Like Cell Pgclc Media, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1x+minimal+essential+medium+nonessential+amino+acids+%28mem+neaa/bio_rxiv__2024__05__31__595485-208-16-44?v=Proteintech
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mem non-essential amino acid (neaa)  (Corning Life Sciences)
90
Corning Life Sciences mem non-essential amino acid (neaa)
a) GSEA of Stag3 KD RNA-seq data reveals a negative normalised enrichment score of -1.50 (adj p= 0.044) for the Spermatogenesis signature (see Methods). b) Relative mRNA expression of PGC regulators in siLuc and siStag3 mESCs. Data is shown as fold-change from UT and is from 9 independent biological replicates. Quantifications and statistical analysis as before. c) Representative brightfield (top row), epi-fluorescent (middle row) and FACS profiles of PRDM1-GFP mESCs at select timepoints during in vitro differentiation into PGCLCs. Scale bar is 200um. d) Relative mRNA expression of select mESC ( Nanog), EpiLC ( Dnmt3b ), <t>PGCLC</t> ( Prdm1, Dppa3 ) and cohesin ( Stag1, 2, 3 and Smc1b ) genes from various timepoints during PGCLC differentiation in vitro . EB GFP+ cells were FACS sorted at the timepoints shown and collected for qRT-PCR. Gene expression is relative to levels in mESCs. Data is from 3 independent biological replicates. e) (Top) Representative brightfield and epi-fluorescent images of DPPA3-GFP mESCs treated with siLuc or siStag3 at three timepoints of embryoid body (EB) differentiation. (Bottom) Representative FACS profile of DPPA3-GFP d6 EB treated with siLuc or siStag3. Scale bar is 200μm. f) Left, quantification of DPPA3-GFP GeoMean or right, the % of DPPA3-GFP+ cells assessed by FACS (relative to UT mESCs) at different stages of PGCLC differentiation and upon siRNA treatment. Data is from 4 independent biological replicates. Quantifications and statistical analysis as before. g) Relative mRNA expression of select genes in mESCs or d6 EB GFP+ cells in siLuc and siStag3 conditions. Data is shown as fold-change from UT and is from 3 independent biological replicates. Quantifications and statistical analysis as above.
Mem Non Essential Amino Acid (Neaa), supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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non-essential amino acids  (Biochrom)
90
Biochrom non-essential amino acids
a) GSEA of Stag3 KD RNA-seq data reveals a negative normalised enrichment score of -1.50 (adj p= 0.044) for the Spermatogenesis signature (see Methods). b) Relative mRNA expression of PGC regulators in siLuc and siStag3 mESCs. Data is shown as fold-change from UT and is from 9 independent biological replicates. Quantifications and statistical analysis as before. c) Representative brightfield (top row), epi-fluorescent (middle row) and FACS profiles of PRDM1-GFP mESCs at select timepoints during in vitro differentiation into PGCLCs. Scale bar is 200um. d) Relative mRNA expression of select mESC ( Nanog), EpiLC ( Dnmt3b ), <t>PGCLC</t> ( Prdm1, Dppa3 ) and cohesin ( Stag1, 2, 3 and Smc1b ) genes from various timepoints during PGCLC differentiation in vitro . EB GFP+ cells were FACS sorted at the timepoints shown and collected for qRT-PCR. Gene expression is relative to levels in mESCs. Data is from 3 independent biological replicates. e) (Top) Representative brightfield and epi-fluorescent images of DPPA3-GFP mESCs treated with siLuc or siStag3 at three timepoints of embryoid body (EB) differentiation. (Bottom) Representative FACS profile of DPPA3-GFP d6 EB treated with siLuc or siStag3. Scale bar is 200μm. f) Left, quantification of DPPA3-GFP GeoMean or right, the % of DPPA3-GFP+ cells assessed by FACS (relative to UT mESCs) at different stages of PGCLC differentiation and upon siRNA treatment. Data is from 4 independent biological replicates. Quantifications and statistical analysis as before. g) Relative mRNA expression of select genes in mESCs or d6 EB GFP+ cells in siLuc and siStag3 conditions. Data is shown as fold-change from UT and is from 3 independent biological replicates. Quantifications and statistical analysis as above.
Non Essential Amino Acids, supplied by Biochrom, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1x+minimal+essential+medium+nonessential+amino+acids+%28mem+neaa/pm32592404-277-48-53?v=Biochrom
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non-essential amino acids (neaa  (FUJIFILM)
90
FUJIFILM non-essential amino acids (neaa
a) GSEA of Stag3 KD RNA-seq data reveals a negative normalised enrichment score of -1.50 (adj p= 0.044) for the Spermatogenesis signature (see Methods). b) Relative mRNA expression of PGC regulators in siLuc and siStag3 mESCs. Data is shown as fold-change from UT and is from 9 independent biological replicates. Quantifications and statistical analysis as before. c) Representative brightfield (top row), epi-fluorescent (middle row) and FACS profiles of PRDM1-GFP mESCs at select timepoints during in vitro differentiation into PGCLCs. Scale bar is 200um. d) Relative mRNA expression of select mESC ( Nanog), EpiLC ( Dnmt3b ), <t>PGCLC</t> ( Prdm1, Dppa3 ) and cohesin ( Stag1, 2, 3 and Smc1b ) genes from various timepoints during PGCLC differentiation in vitro . EB GFP+ cells were FACS sorted at the timepoints shown and collected for qRT-PCR. Gene expression is relative to levels in mESCs. Data is from 3 independent biological replicates. e) (Top) Representative brightfield and epi-fluorescent images of DPPA3-GFP mESCs treated with siLuc or siStag3 at three timepoints of embryoid body (EB) differentiation. (Bottom) Representative FACS profile of DPPA3-GFP d6 EB treated with siLuc or siStag3. Scale bar is 200μm. f) Left, quantification of DPPA3-GFP GeoMean or right, the % of DPPA3-GFP+ cells assessed by FACS (relative to UT mESCs) at different stages of PGCLC differentiation and upon siRNA treatment. Data is from 4 independent biological replicates. Quantifications and statistical analysis as before. g) Relative mRNA expression of select genes in mESCs or d6 EB GFP+ cells in siLuc and siStag3 conditions. Data is shown as fold-change from UT and is from 3 independent biological replicates. Quantifications and statistical analysis as above.
Non Essential Amino Acids (Neaa, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1x+minimal+essential+medium+nonessential+amino+acids+%28mem+neaa/pmc06543182-45-25-30?v=FUJIFILM
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non-essential amino acids (neaa - by Bioz Stars, 2026-08
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hgf  (PeproTech)
90
PeproTech hgf
a) GSEA of Stag3 KD RNA-seq data reveals a negative normalised enrichment score of -1.50 (adj p= 0.044) for the Spermatogenesis signature (see Methods). b) Relative mRNA expression of PGC regulators in siLuc and siStag3 mESCs. Data is shown as fold-change from UT and is from 9 independent biological replicates. Quantifications and statistical analysis as before. c) Representative brightfield (top row), epi-fluorescent (middle row) and FACS profiles of PRDM1-GFP mESCs at select timepoints during in vitro differentiation into PGCLCs. Scale bar is 200um. d) Relative mRNA expression of select mESC ( Nanog), EpiLC ( Dnmt3b ), <t>PGCLC</t> ( Prdm1, Dppa3 ) and cohesin ( Stag1, 2, 3 and Smc1b ) genes from various timepoints during PGCLC differentiation in vitro . EB GFP+ cells were FACS sorted at the timepoints shown and collected for qRT-PCR. Gene expression is relative to levels in mESCs. Data is from 3 independent biological replicates. e) (Top) Representative brightfield and epi-fluorescent images of DPPA3-GFP mESCs treated with siLuc or siStag3 at three timepoints of embryoid body (EB) differentiation. (Bottom) Representative FACS profile of DPPA3-GFP d6 EB treated with siLuc or siStag3. Scale bar is 200μm. f) Left, quantification of DPPA3-GFP GeoMean or right, the % of DPPA3-GFP+ cells assessed by FACS (relative to UT mESCs) at different stages of PGCLC differentiation and upon siRNA treatment. Data is from 4 independent biological replicates. Quantifications and statistical analysis as before. g) Relative mRNA expression of select genes in mESCs or d6 EB GFP+ cells in siLuc and siStag3 conditions. Data is shown as fold-change from UT and is from 3 independent biological replicates. Quantifications and statistical analysis as above.
Hgf, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1x+minimal+essential+medium+nonessential+amino+acids+%28mem+neaa/pmc07363961__mmc1-104-18-19?v=PeproTech
Average 90 stars, based on 1 article reviews
hgf - by Bioz Stars, 2026-08
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medium  (Thermo Fisher)
99
Thermo Fisher medium
a) GSEA of Stag3 KD RNA-seq data reveals a negative normalised enrichment score of -1.50 (adj p= 0.044) for the Spermatogenesis signature (see Methods). b) Relative mRNA expression of PGC regulators in siLuc and siStag3 mESCs. Data is shown as fold-change from UT and is from 9 independent biological replicates. Quantifications and statistical analysis as before. c) Representative brightfield (top row), epi-fluorescent (middle row) and FACS profiles of PRDM1-GFP mESCs at select timepoints during in vitro differentiation into PGCLCs. Scale bar is 200um. d) Relative mRNA expression of select mESC ( Nanog), EpiLC ( Dnmt3b ), <t>PGCLC</t> ( Prdm1, Dppa3 ) and cohesin ( Stag1, 2, 3 and Smc1b ) genes from various timepoints during PGCLC differentiation in vitro . EB GFP+ cells were FACS sorted at the timepoints shown and collected for qRT-PCR. Gene expression is relative to levels in mESCs. Data is from 3 independent biological replicates. e) (Top) Representative brightfield and epi-fluorescent images of DPPA3-GFP mESCs treated with siLuc or siStag3 at three timepoints of embryoid body (EB) differentiation. (Bottom) Representative FACS profile of DPPA3-GFP d6 EB treated with siLuc or siStag3. Scale bar is 200μm. f) Left, quantification of DPPA3-GFP GeoMean or right, the % of DPPA3-GFP+ cells assessed by FACS (relative to UT mESCs) at different stages of PGCLC differentiation and upon siRNA treatment. Data is from 4 independent biological replicates. Quantifications and statistical analysis as before. g) Relative mRNA expression of select genes in mESCs or d6 EB GFP+ cells in siLuc and siStag3 conditions. Data is shown as fold-change from UT and is from 3 independent biological replicates. Quantifications and statistical analysis as above.
Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1x+minimal+essential+medium+nonessential+amino+acids+%28mem+neaa/pmc12528229-206-61-79?v=Thermo+Fisher
Average 99 stars, based on 1 article reviews
medium - by Bioz Stars, 2026-08
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minimum essential medium nonessential amino acids  (Corning Life Sciences)
90
Corning Life Sciences minimum essential medium nonessential amino acids
a) GSEA of Stag3 KD RNA-seq data reveals a negative normalised enrichment score of -1.50 (adj p= 0.044) for the Spermatogenesis signature (see Methods). b) Relative mRNA expression of PGC regulators in siLuc and siStag3 mESCs. Data is shown as fold-change from UT and is from 9 independent biological replicates. Quantifications and statistical analysis as before. c) Representative brightfield (top row), epi-fluorescent (middle row) and FACS profiles of PRDM1-GFP mESCs at select timepoints during in vitro differentiation into PGCLCs. Scale bar is 200um. d) Relative mRNA expression of select mESC ( Nanog), EpiLC ( Dnmt3b ), <t>PGCLC</t> ( Prdm1, Dppa3 ) and cohesin ( Stag1, 2, 3 and Smc1b ) genes from various timepoints during PGCLC differentiation in vitro . EB GFP+ cells were FACS sorted at the timepoints shown and collected for qRT-PCR. Gene expression is relative to levels in mESCs. Data is from 3 independent biological replicates. e) (Top) Representative brightfield and epi-fluorescent images of DPPA3-GFP mESCs treated with siLuc or siStag3 at three timepoints of embryoid body (EB) differentiation. (Bottom) Representative FACS profile of DPPA3-GFP d6 EB treated with siLuc or siStag3. Scale bar is 200μm. f) Left, quantification of DPPA3-GFP GeoMean or right, the % of DPPA3-GFP+ cells assessed by FACS (relative to UT mESCs) at different stages of PGCLC differentiation and upon siRNA treatment. Data is from 4 independent biological replicates. Quantifications and statistical analysis as before. g) Relative mRNA expression of select genes in mESCs or d6 EB GFP+ cells in siLuc and siStag3 conditions. Data is shown as fold-change from UT and is from 3 independent biological replicates. Quantifications and statistical analysis as above.
Minimum Essential Medium Nonessential Amino Acids, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1x+minimal+essential+medium+nonessential+amino+acids+%28mem+neaa/pmc10825048-205-46-53?v=Corning+Life+Sciences
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minimum essential medium nonessential amino acids - by Bioz Stars, 2026-08
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neaa  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc neaa
a) GSEA of Stag3 KD RNA-seq data reveals a negative normalised enrichment score of -1.50 (adj p= 0.044) for the Spermatogenesis signature (see Methods). b) Relative mRNA expression of PGC regulators in siLuc and siStag3 mESCs. Data is shown as fold-change from UT and is from 9 independent biological replicates. Quantifications and statistical analysis as before. c) Representative brightfield (top row), epi-fluorescent (middle row) and FACS profiles of PRDM1-GFP mESCs at select timepoints during in vitro differentiation into PGCLCs. Scale bar is 200um. d) Relative mRNA expression of select mESC ( Nanog), EpiLC ( Dnmt3b ), <t>PGCLC</t> ( Prdm1, Dppa3 ) and cohesin ( Stag1, 2, 3 and Smc1b ) genes from various timepoints during PGCLC differentiation in vitro . EB GFP+ cells were FACS sorted at the timepoints shown and collected for qRT-PCR. Gene expression is relative to levels in mESCs. Data is from 3 independent biological replicates. e) (Top) Representative brightfield and epi-fluorescent images of DPPA3-GFP mESCs treated with siLuc or siStag3 at three timepoints of embryoid body (EB) differentiation. (Bottom) Representative FACS profile of DPPA3-GFP d6 EB treated with siLuc or siStag3. Scale bar is 200μm. f) Left, quantification of DPPA3-GFP GeoMean or right, the % of DPPA3-GFP+ cells assessed by FACS (relative to UT mESCs) at different stages of PGCLC differentiation and upon siRNA treatment. Data is from 4 independent biological replicates. Quantifications and statistical analysis as before. g) Relative mRNA expression of select genes in mESCs or d6 EB GFP+ cells in siLuc and siStag3 conditions. Data is shown as fold-change from UT and is from 3 independent biological replicates. Quantifications and statistical analysis as above.
Neaa, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1x+minimal+essential+medium+nonessential+amino+acids+%28mem+neaa/bio_rxiv__2021__07__12__452054-151-48-46?v=STEMCELL+Technologies+Inc
Average 90 stars, based on 1 article reviews
neaa - by Bioz Stars, 2026-08
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Image Search Results


a) GSEA of Stag3 KD RNA-seq data reveals a negative normalised enrichment score of -1.50 (adj p= 0.044) for the Spermatogenesis signature (see Methods). b) Relative mRNA expression of PGC regulators in siLuc and siStag3 mESCs. Data is shown as fold-change from UT and is from 9 independent biological replicates. Quantifications and statistical analysis as before. c) Representative brightfield (top row), epi-fluorescent (middle row) and FACS profiles of PRDM1-GFP mESCs at select timepoints during in vitro differentiation into PGCLCs. Scale bar is 200um. d) Relative mRNA expression of select mESC ( Nanog), EpiLC ( Dnmt3b ), PGCLC ( Prdm1, Dppa3 ) and cohesin ( Stag1, 2, 3 and Smc1b ) genes from various timepoints during PGCLC differentiation in vitro . EB GFP+ cells were FACS sorted at the timepoints shown and collected for qRT-PCR. Gene expression is relative to levels in mESCs. Data is from 3 independent biological replicates. e) (Top) Representative brightfield and epi-fluorescent images of DPPA3-GFP mESCs treated with siLuc or siStag3 at three timepoints of embryoid body (EB) differentiation. (Bottom) Representative FACS profile of DPPA3-GFP d6 EB treated with siLuc or siStag3. Scale bar is 200μm. f) Left, quantification of DPPA3-GFP GeoMean or right, the % of DPPA3-GFP+ cells assessed by FACS (relative to UT mESCs) at different stages of PGCLC differentiation and upon siRNA treatment. Data is from 4 independent biological replicates. Quantifications and statistical analysis as before. g) Relative mRNA expression of select genes in mESCs or d6 EB GFP+ cells in siLuc and siStag3 conditions. Data is shown as fold-change from UT and is from 3 independent biological replicates. Quantifications and statistical analysis as above.

Journal: bioRxiv

Article Title: STAG3 promotes exit from pluripotency through post-transcriptional mRNA regulation in the cytoplasm

doi: 10.1101/2024.05.31.595485

Figure Lengend Snippet: a) GSEA of Stag3 KD RNA-seq data reveals a negative normalised enrichment score of -1.50 (adj p= 0.044) for the Spermatogenesis signature (see Methods). b) Relative mRNA expression of PGC regulators in siLuc and siStag3 mESCs. Data is shown as fold-change from UT and is from 9 independent biological replicates. Quantifications and statistical analysis as before. c) Representative brightfield (top row), epi-fluorescent (middle row) and FACS profiles of PRDM1-GFP mESCs at select timepoints during in vitro differentiation into PGCLCs. Scale bar is 200um. d) Relative mRNA expression of select mESC ( Nanog), EpiLC ( Dnmt3b ), PGCLC ( Prdm1, Dppa3 ) and cohesin ( Stag1, 2, 3 and Smc1b ) genes from various timepoints during PGCLC differentiation in vitro . EB GFP+ cells were FACS sorted at the timepoints shown and collected for qRT-PCR. Gene expression is relative to levels in mESCs. Data is from 3 independent biological replicates. e) (Top) Representative brightfield and epi-fluorescent images of DPPA3-GFP mESCs treated with siLuc or siStag3 at three timepoints of embryoid body (EB) differentiation. (Bottom) Representative FACS profile of DPPA3-GFP d6 EB treated with siLuc or siStag3. Scale bar is 200μm. f) Left, quantification of DPPA3-GFP GeoMean or right, the % of DPPA3-GFP+ cells assessed by FACS (relative to UT mESCs) at different stages of PGCLC differentiation and upon siRNA treatment. Data is from 4 independent biological replicates. Quantifications and statistical analysis as before. g) Relative mRNA expression of select genes in mESCs or d6 EB GFP+ cells in siLuc and siStag3 conditions. Data is shown as fold-change from UT and is from 3 independent biological replicates. Quantifications and statistical analysis as above.

Article Snippet: At 48h post-EpiLC induction, cells were seeded at a density of 4x10 3 cells/well in 50μl Primordial germ cell-like cell (PGCLC) media (GMEM 15% KnockOut serum replacement, 1mM Sodium Pyruvate, 1X NEAA, 2mM Glutamax, 1X PenStrep, 50mM BMe) supplemented with human recombinant BMP4 (500ng/ml; Proteintech HZ-1045) and mouse recombinant LIF (1000U/ml).

Techniques: RNA Sequencing, Expressing, In Vitro, Quantitative RT-PCR, Gene Expression